Crude Sample Analysis

Attana systems combined with the Attana IgG capture kits provide the ability to capture antibodies directly from crude samples, thus facilitating the study of antibodies.

The characterization of antibody-antigen interactions has traditionally been very time consuming since antibodies need to be purified and sometimes labelled prior to studies. Additionally, these steps are typically associated with problems such as optimization and yield.

The Attana systems together with the Attana IgG capture kits offer a new solution saving time and eliminating potential issues. Enabling the capture of antibodies directly from crude samples the Attana systems facilitate interaction studies of antibodies.

Attana Benefits

• A real time, label-free method to capture mouse IgG from hybridoma supernatants which also can be replicated for capture of human IgGs.
• Kinetic information about the interactions, association (kon) and dissociation (koff) rate constants can be derived to calculate the affinity (KD) between the molecules.
• Facilitates rapid screening of up to 192 antibody-antigen pairs directly from crude samples, such as hybridoma supernatants, without the necessity to purify or label the antibody.
• Saves time and reduces cost, as generic immobilization and regeneration conditions have been established and prior purification of the antibody is unnecessary.