Epitope Mapping

Immunological reagents with optimal recognition of antigen in solution are essential for immunoassay development. Recognition of independent epitopes and good kinetic properties are additionally important parts of a selection process for identification of optimal monoclonal antibodies for immunoassay development.

In the selection process for identification of optimal monoclonal antibodies for immunoassay development, analyses are typically performed using different immunochemical methods including cross-inhibition studies, determination of dose-response curves (for all possible antibody-antigen combinations) and analysis of binding constants. The Attana systems enable real time, label-free analysis of antibody–antigen binding characteristics and provides kinetic data on the interaction.

Attana Benefits

• Allows affinity ranking of antibodies and early identification of reagents with good kinetic characteristics.
• Early evaluation and selection of suitable antibody pairs for the design of sandwich immunoassays and for epitope mapping.
• Saves time compared to other immunochemical methods including cross-inhibition studies and permits the determination of dose-response curves and kinetic parameters.
• Cost efficient characterization of antibody-antigen interactions.