attana - sensor technologies

New scientific publication

The researchers at the Linneus University, Centre for Biomaterials Chemistry, S. Suriyanarayanan, I. Nicholls et. al. have their latest Attana related work accepted for publication in the Journal of Colloid and Interface Science.

The paper, Protein-resistant hyperbranched polyethyleneimine brush surfaces describes a novel design of a hyperbranched PEI modified gold surface that has been investigated with respect to its ability to resist non-specific adsorption of proteins and involves immobilization of PEI to gold surfaces.  The hyperbranched polymer brushes were characterized by X-ray photoelectron spectroscopy (XPS). Reflection absorption infrared spectroscopy (RAIRS) and ellipsometry measurements showed the thickness of the PEI brushes increases with adsorption solution ionic strength. Protein adsorption was evaluated under flow injection analysis (FIA) conditions using a quartz crystal microbalance (Attana A100 system). The PEI brushes suppress protein adsorption, for example, cytochrome C, bovine serum albumin, and ribonuclease A, to less than 0.08 lg/cm2 and the protein resistance increases with increasing ionic strength of the carrier solution, performance comparable to that achieved with comparable PEG-coated surfaces. The PEI brushes were exceptionally stable, with adsorption characteristics maintained after 6 months storage in aqueous conditions.

To read the full abstract, follow the link and click on the article.