attana - sensor technologies

Cell-based assay

The label-free cell-based biosensor, Attana Cell 200, allows in situ evaluation of binding characteristics where uncleared crude and serum containing samples can be directly analyzed at the cell surface. By studying target interactions in the native environment, only candidates with biologically relevant properties will be selected for downstream applications generating a faster and cheaper track to drugability.

Interactions those were previously just possible to study at the direct macromolecular level can now be analyzed label-free in the native biological context at the cell surface. This can be studied with crude or serum containing samples, in real time, under a continuous flow and in a temperature controlled environment all to obtain a surrounding as close as possible to the in vivo environment within the biosensor, getting the real specificity of the reaction in the native environment early in the discovery process. 

All kinds of macromolecular interactions to binding partners at the cell surface can easily be analyzed with the Attana Cell 200 biosensor. Using Attana’s proprietary cell specific COP-1 surface coating, cells will be growing in a tissue culture flask-like environment at the sensor chip, displaying their true biological surface expression profile and morphology. To characterize binding events from both a biochemical and a cell-based assay is essential in order to fully understand the details of the underlying biological mechanism at the molecular level, which can be done with the “one-stop-shop system” of Attana.

The full binding kinetics (ka and kd) and affinity (KD) is provided using the user-friendly and intuitive Attaché-software and/or TraceDrawer software for analyzing collected data.

Added features utilizing cell-based kinetics assays are biologically relevant information such as surface target accessibility, avidity and drugability. Further, potentially detrimental off-target interactions can easily be identified during a first screening procedure allowing for only the correct candidates to proceed to the next step in the process and eliminate those candidates of poor quality. All-in-all providing means earlier during a discovery process detect candidates that are to fail in later phases, in the end saving time and money.