attana - sensor technologies

Scientific Posters

  • Antibody Engineering and Therapeutics Conference - The Annual Meeting of the Antibody Society, Dec. 8-12, 2013, Huntington Beach, CA, USA Cell-Antibody Interaction Study Using Herceptin™ (trastuzumab) Binding to SKOV-3 Epithelial Cancer Cells Characterized by Attana™ Quartz Crystal Microbalance TechnologyL. Elmlund, C. Käck, L. Kaiser, T. Aastrup, I.A. Nicholls
    Abstract

    Overexpressed HER-2 (human epidermal growth factor 2) receptor is a problem in many cancer tumors. In fact, 20-30% of breast cancer tumors are caused by HER-2 receptor overexpression and the receptor is therefore used both as a biomarker and for treatment. Because of its poor prognosis early diagnosis of these tumors is of importance.

  • Poster presented at Asian Communication for Glycobiology and Glycotechnology, Oct 2012 Cell-Based Biosensors: A Quartz Crystal Microbalance Approach to the Study of Carbohydrate-LectinInteractionsZhichaoPei, JulienSaint-Guirons, Camilla Käck, Bjorn Ingemarsson2, TeodorAastrup
  • ICS conference Sep 2012, Poland Searching by Affinity Ranking for the IC2 Monoclonal Autoantibody Targeted Autoantigenic Epitope on the Surface of Pancreatic Beta-CellsIda Dalgaard Pedersen, Kathrine Louise Jensen, Camilla Käck, Theodor Aastrup, Mickael Blaise, Anna Åkesson.
    Abstract

    Background:

    Type 1 diabetes mellitus is a chronic disorder resulting from autoimmune destruction of insulin-producing pancreatic β-cells. In advance of clinical symptoms, autoantibodies are produced and self-reactive lymphocytes infiltrate the pancreas to destroy β-cells.

    Results:

    Affinity measurements using QCM revealed a very high cellular affinity. Furthermore, binding of different formats of IC2 were measured to intact INS-1E cells, sonicated plasma membrane vesicles, and lipids, using the Q-Sense E4 quartz crystal
    microbalance with dissipation.

  • A biosensor-based concept for antibody-GPCR interaction studies using immobilized lipoparticlesSamuel Altun, Magnus Brändén, Teodor Aastrup, Torbjörn Pettersson
    Abstract

    This study demonstrates the applicability of the Attana A200 biosensor in combination with memLAYER immobilization for functional characterization of membrane proteins, such as GPCRs. Using the lipoparticle system the often time-consuming and expensive process of i) purifyingand ii) reconstituting the membrane protein of interest into liposomes for functional studies of membrane proteins are circumvented. The results also illustrate the benefits of immobilizing multiplelayers of lipoparticles using memLAYER, which increases the sensor surface density of the receptor and the response in subsequent ligand-receptor binding events.

  • August 19, 2013 ATROSAB, a humanized antagonistic anti-tumor necrosis factor receptor one specific antibodyKirstiin A Zettlitz, Verena Lorenz, Peter Scheurich, Klaus Pfizenmaier, Andreas Herrmann & Roland E. Kontermann.
  • 2007 Flow Injection Assay of the Path ogenic Bacteria Using Lectin-based QCM BiosensorGulnara Safina, Margret van Lier and Bengt Danielsson, Pure and Applied Biochemistry, Chemical Center.
    Abstract

    A flow injection assay for the detection and identification of the pathogenic bacteria strains using lectin-based QCM biosensor has been proposed